| Other Notes | Handle the toxin gently. Do not vortex. Use sufficient volume of buffer to wash down the walls and stopper. Read all handling information found on MSDS prior to reconstitution. |
| Preparation Note | When toxin is used for in vitro studies, it is preincubated in a buffer containing 5 mM dithiothreitol for 30 minutes in order to reduce and thereby activate the toxin. |
| Reconstitution | Reconstitute each 100 μg vial with 100 μL of appropriate buffer. Upon reconstitution, the vial will contain 20 mM HEPES and 1.25% lactose. To insure full recovery of toxin from the vial, include 1 mg/mL BSA in the reconstitution buffer. |
| Physical form | Lyophilized powder containing 20 mM HEPES and 1.25% lactose when reconstituted with the volume of water or buffer indicated on the Certifcate of Analysis. |
| Biochem/physiol Actions | Neurotoxin that inhibits release of catecholamines from adrenal medulla; blocks release of acetylcholine at neuromuscular junctions. |
